Microarray

The EUROIMMUN EUROArray system combines the advantages of the polymerase chain reaction (PCR) and microarray technologies for the detection of genetic markers and direct detection of pathogens. The EUROArrays are based on BIOCHIP technology with specific DNA probes. Each BIOCHIP consists of 72 DNA spots and allows duplicate determinations of up to 36 different DNA sequences, including controls. Each EUROArray slide contains five test fields, enabling up to five samples to be analysed in parallel. Several BIOCHIPs may be coated on one field in order to increase the number of detectable DNA sequences.

Technology

For some parameters, EUROIMMUN offers a direct procedure in which the DNA does not need to be isolated from the sample beforehand. The blood is incubated with the extraction solutions included in the kit and can then be used directly in the PCR. With all other parameters, the DNA is obtained conventionally, using a DNA isolation kit. All PCR reagents supplied in EUROArray kits are ready to use, including the DNA polymerase and the validated specific primers. The pre-prepared PCR reagents are just combined, and the DNA is then added to this master mix. Using PCR, the specific genetic sequences of the patient DNA are amplified and the PCR products labelled with a fluorescence dye during their formation. In the hybridisation (incubation) of the PCR products with the EUROArray, these bind to the complementary probes of the EUROArray. The DNA microarray hybridisation is performed under exact, standardised conditions using the proven TITERPLANE technology. After the reading of the EUROArray in the EUROArrayScanner, the fluorescence signals are evaluated fully automatically using the EUROArrayScan software.

Direct procedure

For the majority of human genetic parameters, EUROIMMUN offers the Direct procedure, in which DNA needs not be isolated from the sample beforehand. The blood is incubated with the extraction solutions supplied in the kit and can then be used directly for the PCR.

With all other parameters, the DNA is retrieved using a conventional DNA isolation kit.